Snake venoms comprise mixtures of enzymes and proteins that act on vital systems of the victim. The present study aimed to investigate the histochemical and biochemical effects of the horned viper (Cerastes cerastes gasperetti: Ccg) snake venom in mice. Thirty six male adult Swiss albino mice (20–25g) were injected intraperitoneally and divided into 2 groups (n=18): control group injected with 200 μl saline solution and group (2) injected with LD50 of Ccg venom (0.978 mg/kg). Liver, kidney and testis were collected from six mice after 1, 3 and 6 hours, post envenomation. Histological examination of testis sections after1, 3 and 6 hours, post envenomation showed seminferous tubules displaying an impairment of spermatogenesis stages arrangement with severe vacuolization of most affected tubules and appearance of pyknotic spermatocyt. Histochemical investigations revealed marked reduction in both carbohydrates and proteins after injection of LD50 of Ccg crude venom at the different time. Oxidative stress biomarkers malondialdehyde (MDA) and nitrite/nitrate levels, antioxidants glutathione (GSH) content and catalase (CAT) activity were assayed in tissues homogenates. The venom induced significant increases in the levels of MDA and nitrite/nitrate while the content of GSH and the activity of CAT were significantly decreased, especially after 6 hours of envenomation. The results revealed that the Ccg venom induced time-dependant significant increases in the levels of MDA and nitrite/nitrate in all examined organs, especially in the hepatic tissues. It can be concluded that cytotoxic effects of Ccg venom might be related to induction of excessive oxidative stress and histochemical alternations in organs of the envenomated animals. [Al-Sadoon MK, Abdel Moneim AE, Bauomy AA, Diab MM. Histochemical and Biolchemical effects induced by LD50 of Cerastes cerastes gasperetti crude venom in mice. Life Sci J 2013;10(4):810-817] (ISSN:1097-8135).  http://www.lifesciencesite.com. 102