Snakebite remains an important medical problem in both developing and developed countries because; it is an important cause of mortality and morbidity. Viper snakes are widely distributed in desert areas; the Cerastes cerastes gasperetti is one member of the family Viperidae where their bites are a serious threat to life and since Cerastes cerastes gasperetti venom has received little interest, this study was designed to investigate the effect of LD50 of Cerastes cerastes gasperetti crude venom on histopathological changes as well as on some important serum and tissue homogenates (liver and kidney) parameters. In addition, Cerastes cerastes gasperetti crude venom caused hepatic and renal injuries as indicated by histopathological changes in the liver and kidney tissues. Serum ALT, AST activities, total bilirubin, creatinine and blood urea nitrogen levels were all increased. On contrary, serum levels of ALP, γ-GT, albumin, uric acid and glucose were decreased as a result of envenomation. Kidney and liver lipid profile levels, glycogen content in kidney were also decreased, in contrast, glycogen content in liver and LDH activity in renal and hepatic homogenates expressed an increment as a result. On the basis of the above results it can hypothesize that Cerastes cerastes gasperetti crude venom is potent toxin-mediated hepatorenal toxicity and causes disturbance in carbohydrates and lipids metabolism.