Captive reproduction of Lachesis muta muta was achieved in the ’80s at the Dallas Zoo (Boyer et al., 1989). Ripa (1994) published an account of the reproduction of the Central American species Lachesis stenophrys and Lachesis melanocephala. In this paper we present our experience in the reproduction of Atlantic bushmasters in captivity for the first time, involving parental (male and female) DNA from two (maybe three) different couples.